CANDIDA NORVEGENSIS PDF

Biochemical-phenotypic identification. Particular attention was paid to the esculin hydrolysis, the unique trait thought to be able to distinguish between C. The percentage of identification based on the relative similarity between the biochemical profile of the tested strain and the analyzed profiles was recorded. It is worth noting that P. Bruker Biotyper version 2. Bruker database version 2.

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Biochemical-phenotypic identification. Particular attention was paid to the esculin hydrolysis, the unique trait thought to be able to distinguish between C. The percentage of identification based on the relative similarity between the biochemical profile of the tested strain and the analyzed profiles was recorded.

It is worth noting that P. Bruker Biotyper version 2. Bruker database version 2. Neither P. Molecular analysis. Direct sequencing was performed using a BigDye Terminator V3. Chromatograms were then edited and manually corrected using BioEdit software version 7. Cloning of the rDNA intergenic spacer region. Because the chromatograms of the ITS region obtained through direct sequencing frequently resulted in superimposed traces, we decided to clone those amplicons before sequencing.

For each amplicon i. Phylogenetic analysis. Sequences were aligned using Clustal X software version 2. Sexual reproduction. In order to investigate the potential sexual reproduction of C. Strains were spotted individually on the potassium acetate plates but also in combination with every other strain used in this study.

As it readily produced asci, P. Each day, a thin smear of the culture was stained using the Wirtz stain that distinguishes the blastoconidia stained in pink from the ascospores in green Nucleotide sequence accession numbers.

Traces for one clinical isolate SA and one P. In contrast, for six strains, a complete chromatogram was obtained. The sequence obtained from the P. Five reference strains and 11 clinical isolates presented superimposed chromatograms that were partially interpretable. Sharp analysis of these chromatograms suggested the superimposition of multiple staggered traces see Fig. S1 in the supplemental material. Thus, ITS amplicons from 5 P. Unambiguous chromatograms were obtained in all cases.

One to three different alleles were identified per strain, in accordance with the superimposed traces obtained from direct sequencing Fig.

HAROLD GARFINKEL ETNOMETODOLOGIA PDF

Candida norvegensis: a fluconazole-resistant species.

Rev Iberoam Micol. Epub May 2. Candida norvegensis fungemia in a liver transplant recipient. Electronic address: amoreno clinic. The use of fluconazole as antifungal prophylaxis has been described as a risk factor for the development of infections by fluconazole resistant Candida strains. We report a case of Candida norvegensis bloodstream infection in a liver transplant recipient. CASE REPORT: A year-old man, who received a third liver allograft and became worse with the onset of ischemic cholangiopathy and recurrent episodes of cholangitis, was admitted to our hospital due to the development of intra-abdominal abscesses.

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Candida norvegensis fungemia in a liver transplant recipient.

Conflict of interests: the authors declare no potential conflict of interests. Musso et al. This article has been cited by other articles in PMC. Abstract Candida norvegensis is an emerging fluconazole-resistant pathogen isolated in most cases from skin and mucous membranes of immunocompromized patients. Documented invasive candidiasis IC due to C. We report a liver transplant patient who developed IC due to C.

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